ZFIN Image: Ota et al., 2009, Fig. S2

Image

Figure Caption

Fig. S2 Evaluation of the FGF signal in embryos injected with mRNA for dominant-negative FGFRs and/or fgf genes. (A–H) The endogenous expression of spry4 in the anterior neural boundary (amb), MHB, and tailbud (tb) (A) was downregulated by overexpression of mdn-R genes (B–F), XFD (G), and sdn-R3 (H) at the bud stage. (I–N) Ectopic expression of spry4 induced by fgf3 and fgf8 was suppressed efficiently by mdn-R1, whereas sdn-R3 abrogated the effects of fgf8, but not of fgf3. The apparent reduction of spry4 expression in the embryo co-expressing fgf3 and sdn-R3 is probably due to the suppression of endogenous FGF8 subfamily genes. Lateral views with anterior to the top and dorsal to the right.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 126(1-2), Ota, S., Tonou-Fujimori, N., and Yamasu, K., The roles of the FGF signal in zebrafish embryos analyzed using constitutive activation and dominant-negative suppression of different FGF receptors, 1-17, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.