ZFIN Image: Sittaramane et al., 2009, Fig. 6

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Figure Caption

Fig. 6 Genetic interactions between stbm and lama1. All panels show dorsal views of the hindbrain with anterior to the left. Tg(isl1:gfp) embryos were fixed at 48 hpf, and processed for immunohistochemistry with zn5 antibody (red) to label dorsal commissural neurons and axons at rhombomere boundaries (A–F), and anti-GFP antibody (green) to label FBMNs (A–H; arrowheads). (A) FBMNs migrate normally in a control embryo. (B, C) Partial loss of FBMN migration in embryos injected with suboptimal dose of lama1 MO (B) or stbm MO (C). (D) Complete loss of FBMN migration in an embryo injected with suboptimal doses of lama1 and stbm MOs. (E) Partial loss of FBMN migration in a trilobite^tc240a (tri) heterozygous (stbm +/-) embryo. (F) Complete loss of FBMN migration in a trilobite heterozygote injected with suboptimal dose of lama1 MO. (G) Greatly reduced FBMN migration in a bashful^b765 homozygous (lama1 -/-) embryo. (H) Complete loss of FBMN migration in a trilobite; bashful double heterozygote. Scale bar in H (75 μm for A–H).

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Acknowledgments

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Reprinted from Developmental Biology, 325(2), Sittaramane, V., Sawant, A., Wolman, M.A., Maves, L., Halloran, M.C., and Chandrasekhar, A., The cell adhesion molecule Tag1, transmembrane protein Stbm/Vangl2, and Lamininalpha1 exhibit genetic interactions during migration of facial branchiomotor neurons in zebrafish, 363-373, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.