Fig. S2

Fig. S2 Transient expression of pufferfish evx1:GFP reporter constructs in zebrafish embryos. All reporter constructs contained a 7 kb promoter and GFP fused in frame in exon 2 as described in Fig. 2A. Constructs with or without the 3′ evx1 region (- 3′ evx1 and + 3′ evx1) were injected into the cytoplasm of one-cell stage embryos. Fluorescence images of whole-mount living embryos were taken under a dissection microscope at ∼ 32 hpf. The region of the ‘spinal cord’ examined in our study is labeled in A and the dorso-ventral limits of the spinal cord marked by dash lines. Constructs 7 k (7 kb promoter alone), 10k, 5035F, 2933F and 1917F are described in Fig. 2A. Dorsal is up and rostral is to the left.