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Fig. 3 The effect of loss of Notch signaling on surface accumulation of DeltaD. DeltaD distribution in DMSO-treated (A,A′), DAPT-treated (B,B′) or DN-Su(H)-expressing zebrafish embryos (C,C′) at the tail bud stage, and in DMSO-treated (D,D′) and DAPT-treated (E,E′)embryos at the six-somite stage (6 ss). Areas shown in A-C′ correspond approximately to rectangular areas in Fig. S1E,F (see supplementary material). Embryos were stained with zdD2 (A-E) and double-stained (A′-E′) with β-catenin (zdD2 in green, β-catenin in red). DAPT and DN-Su(H) treatment increased the numbers of cells expressing DeltaD but did not result in DeltaD accumulation at the cell surface at the tail bud stage. Some cell surface DeltaD is detected in DAPT-treated embryos by the six-somite stage; however, it is accompanied by intense cytoplasmic puncta.