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Fig. S1 Distribution of neutrophils, macrophages, and mmp9-positive cells during larval and adult regeneration. (A) ISH analyses for expression of mpx, a neutrophils marker (top row), L-plastin, a macrophage marker (middle row) and mmp9 (bottom row) during larval fin fold regeneration. (B) Expression of mpx, L-plastin and mmp9 during adult fin regeneration. Only a few mpx-positive neutrophils were seen during regeneration up to 24 hpa (top row), whereas a large number of macrophages (middle row) were recruited to regions around the wound extending over 1 ray segment away from the stump. The mmp9-positive cells appeared by 24 hpa, however many mmp9-positive cells were localized at the amputation site and adjacent inter-ray regions with apparently different expression profile from mpx or L-plastin. The same magnifications for respective panels in A and B (scale bars in top left panels).
Reprinted from Developmental Biology, 325(1), Yoshinari, N., Ishida, T., Kudo, A., and Kawakami, A., Gene expression and functional analysis of zebrafish larval fin fold regeneration, 71-81, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.