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Fig. 5 lpp mRNA levels are reduced in Wnt11 morphants and in embryos overexpressing Wnt11 or a dominant negative form of Rho kinase 2. Zebrafish embryos were injected with Wnt11-MO (2 ng), wnt11 RNA (100 pg) or dnRok2 RNA (100 pg). Embryos showing clear C&E defects at tailbud stage were selected and total RNA was extracted. Quantitative RT-PCR with primers for lpp showed reduced levels of lpp mRNA in all three conditions (*, p < 0.05; **, p < 0.01). n, total number of embryos per condition from four independent experiments.
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Reprinted from Developmental Biology, 320(1), Vervenne, H.B., Crombez, K.R., Lambaerts, K., Carvalho, L., Koeppen, M., Heisenberg, C.P., Van de Ven, W.J., and Petit, M.M., Lpp is involved in Wnt/PCP signaling and acts together with Scrib to mediate convergence and extension movements during zebrafish gastrulation, 267-277, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.