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Fig. S6
Procedure for Fate Mapping at the 8 Somite Stage
(A) 5-10 cells within the LPM were labeled, and their position relative to the tip of the notochord was recorded. Image is an overlay from a representative experiment in which cells were labeled at 70 ncds.
(B) At 48 hpf, progeny of labeled cells are detected.
(C) WT embryo exhibiting labeled cells in pectoral fin. Presumptive fin cartilage (yellow arrow) was scored as fin, while fin ectoderm (black arrow) was not.
(D) BMS-treated embryo exhibiting labeled cardiomyocytes (arrows).
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Reprinted from Developmental Cell, 15(6), Waxman, J.S., Keegan, B.R., Roberts, R.W., Poss, K.D., and Yelon, D., Hoxb5b acts downstream of retinoic Acid signaling in the forelimb field to restrict heart field potential in zebrafish, 923-934, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell