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Fig. 4 Abnormal behavioral phenotype and impaired development of spinal neural network of Ap1s1 knockdown zebrafish.
Consecutive images from films illustrating the response to touch in 48 hpf wild type (WT) (A) and Ap1s1 KD larvae (F). The WT larva reacted to touch by swimming away. In contrast, the Ap1s1 KD larvae exhibit slow and impaired reaction. Immunostaining in wholemount 48 hpf WT (B–E) and Ap1s1 KD larvae (G–J) illustrating the reduced axonal labeling (anti-acetylated tubulin; B and G red, the arrows point to the ventral roots), the reduced number of newly born cells (anti-HU antibody; red in C and H), and the number of interneurons (anti-Pax2 antibody; E and J green) in the Ap1s1 KD larvae. In turn, the number of motoneurons (anti-HB9 antibody; D and I, red) was similar both in WT and Ap1s1 KD. Scale bars: 25 μM.