Fig. 8

Fig. 8 Apical localization of the other Pard6 family members and partial rescue of the pard6γb^s441 mutant phenotype by Pard6α. (A, C, E, G, I, K) Optical sections through the dorsal neural tube of live 24 hpf embryos between the first and sixth somite. (B, D, F, H, J, L) Transverse sections through the neural tube between the first and sixth somite of 24 hpf embryos, stained for filamentous actin. (A–D) Embryos injected with mRNA encoding Pard6γa-GFP. (A, B) In wildtype embryos, Pard6γa-GFP localized to the apical membranes. (panels C–D) In pard6γb^s441 mutant embryos, Pard6γa-GFP localized to the discontinuous apical membranes, and it did not rescue the s441 mutant phenotype. (E–H) Embryos injected with mRNA encoding Pard6β-mCh. (E, F) In wildtype embryos, Pard6β-mCh localized to the apical membranes. (G, H) in pard6γb^s441 mutant embryos, Pard6β-mCh localized to the discontinuous apical membranes, and it did not rescue the pard6γb^s441 mutant phenotype. (I–L) Embryos injected with mRNA encoding Pard6α-GFP. (I, J) In wildtype embryos, Pard6α-GFP localized to the apical membranes. (K, L) In pard6γb^s441 mutant embryos, Pard6α-GFP localized to the apical membranes. (K) In this pard6γb^s441 mutant embryo, midline Pard6α-GFP localization was discontinuous and Pard6α-GFP failed to rescue the neurocoel phenotype. (L) In another, and genotyped, pard6γb^s441 mutant embryo, midline Pard6α-GFP localization was continuous and Pard6α-GFP rescued the neurocoel phenotype. Scale bars represent 50 μm.