ZFIN Image: Munson et al., 2008, Fig. 6

Image

Figure Caption

Fig. 6 The PB1, CRIB and PDZ domains are required for Pard6γb function but not localization. (A–E) Live, 24 hpf embryos injected with h2b-mRFP and pard6γb(Δpb1)-GFP (abbreviated ΔPB1-GFP), pard6γb(Δcrib)-GFP (abbreviated ΔCRIB-GFP) or pard6γb(kplg)-GFP (abbreviated KPLG-GFP) mRNA as indicated (dorsal views between the first and sixth somite, anterior to the top). (A–C) In wildtype neural tubes, nuclei are organized on either side of the midline. (D-F) In pard6γb^s441 mutant neural tubes, nuclei are disorganized. In wildtype embryos, ΔPB1-GFP (A), ΔCRIB-GFP (B) and KPLG-GFP (C) localized to the cytoplasm and apical membranes. In pard6γb^s441 mutant embryos, ΔPB1-GFP (D), ΔCRIB-GFP (E) and KPLG-GFP (F) failed to rescue the mutant phenotype and localized to the discontinuous apical membranes. Scale bars represent 50 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 324(1), Munson, C., Huisken, J., Bit-Avragim, N., Kuo, T., Dong, P.D., Ober, E.A., Verkade, H., Abdelilah-Seyfried, S., and Stainier, D.Y., Regulation of neurocoel morphogenesis by Pard6gammab, 41-54, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.