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Fig. S4 Ventral margin extirpation. (A) Model of the procedure. Approximately 100 cells were removed from the ventral margin (red) of 80% epiboly embryos. All images shown are of embryos with their ventral margins removed. (B-D) flh (B), ntl (C) and admp (D) expression in embryos at 90% epiboly. (E-E″) Antibodies against PSMAD1/5/8 (red) and BrdU (green) in longitudinal sections of the tail of 14-somite stage embryos. (F) High-magnification view of notochord cells of 24 hpf embryos at the level of the cloaca. (G,H) shha and ptc1 expression in 24 hpf.
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