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Fig. S5 BrdU incorporation of embryos treated with the cell cycle inhibitors hydroxyurea and aphidicolin. Embryos were treated from 80% epiboly to bud stage, then inhibitors were removed by washing. (A,B) BrdU incorporation of DMSO- (A) and hydroxyurea/aphidicolin-treated (B) bud stage embryos. (C-C″) Antibodies against PSMAD1/5/8 (red) and BrdU (green) in longitudinal sections of the tail of 14-somite hydroxyurea/aphidicolin treated embryos. (D,E) BrdU incorporation of DMSO- (D) and hydroxyurea/aphidicolin-treated (E) 24 hpf embryos.
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