ZFIN Image: Martin et al., 1999, Fig. 7

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Figure Caption

Fig. 7 Treatment of zebrafish embryos with 5-azacytidine in vivo results in reduced DNA methylation. DNA collected from 24-h wild-type embryos, and from 5-azaC-treated embryos were digested with HpaII or MspI, Southern transferred and probed with the DANA/mermaid interspersed repetitive DNA sequence as described under Materials and Methods. This revealed an approximately 50% higher relative abundance of low-molecular-weight fragments in HpaII digested DNA from 5-azaC-treated embryos (lane 1) compared to untreated embryos (lane 3), indicating DNA hypomethylation in treated embryos. DNA collected from embryos treated with 6-azaC (lane 2) show a methylation level similar to that observed from untreated embryo DNA. Densitometric analysis was performed as described in the legend for Fig. 4. Ratios are indicated as the average of 3 independent gels ± SD.

Acknowledgments

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Reprinted from Developmental Biology, 206, Martin, C.C., Laforest, L., Akimenko, M.-A., and Ekker, M., A role for DNA methylation in gastrulation and somite patterning, 189-205, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.