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Fig. 9 Hedgehog signaling is required cell autonomously for effective contribution to myocardium. (A) Transplantation results. Expression of Tg(cmlc2:egfp) in host embryos at 48 hpf indicated myocardial contribution. A ratio of hosts with donor-derived cardiomyocytes to all host embryos screened was used to determine myocardial contribution frequency. Asterisk indicates a statistically significant difference from wild type (P=0.0346, normal approximation of the χ2 test). (B) Wild-type donor-derived cells expressing Tg(cmlc2:egfp) in wild-type host myocardium at 48 hpf. (C) Superimposed bright-field and fluorescent images. Scale bar: 100 μm. (D) MZsmo donor-derived cardiomyocyts expressing Tg(cmlc2:egfp) in wild-type host myocardium. (E) Superimposed bright-field and fluorescent images. (F,G) Wild-type and MZsmo donor-derived skeletal fast muscle cells marked with fluorescein lineage tracer in wild-type host. Scale bar: 100 μm. (H) Donor cell incorporation into skeletal fast muscle lineage, scored at 48 hpf.