Fig. 10

Fig. 10 Defects in peripheral nervous system organization in puma mutants. Staining for nerves and sox10 expression in wild-type (A, C, E) and puma mutant (B, D, F) larvae. All images are cross sections. (A) In wild-type larvae, sox10⁺ cells (blue arrow) can be observed along peripheral nerves and neurites stained for acetylated tubulin (red arrow), consistent with these cells being glia. (B) In puma mutants, residual sox10⁺ cells apparent in whole mounts (e.g., Fig. 9F) also can be observed in close association with nerves and neurites. (C) At late stages of pigment pattern metamorphosis in wild-type, the posterior lateral line nerve is cohesive and well organized (red arrow) and is surrounded by slow muscle fibers of the myotome. Blue staining for sox10 can be seen nearby as well. (D) In puma mutants at the same stage, the lateral line nerve appears disorganized and bundles of fibers can be seen both in the normal position (left red arrow) and medially (right red arrow). At this later stage, more sox10⁺ cells are observed as compared with early metamorphic stages (e.g., Fig. 9B). Arrowhead indicates a melanophore. (E) In a wild-type larva stained only for acetylated tubulin, the lateral line nerve is a solid rod of nerve fibers (arrow). e, epidermis. m, myotome. (F) In puma mutants, the nerve is disorganized and fewer nerve fibers are present (red arrows). Scale bars, (A, B) 10 μm; (C, D) 20 μm; (E, F) 20 μm.