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Fig. 5

ID
ZDB-IMAGE-081021-77
Source
Figures for Malicki et al., 2003
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Figure Caption

Fig. 5 Protein product localization in mutant retinae. At 24 hpf, N-cadherin polypeptide is present in wild-type (A), glom117 (B) but not in pactm101b (C) retinae. This situation persists at later stages of development as shown for the wild-type (D), glom117 mutants (E), and pactm101b mutants (F). Residual staining present in (C) and (F) is most likely due to background fluorescence. As reported previously in wild-type animals, N-cadherin is expressed in plexiform layers (arrows in D and G) and in the optic nerve (arrowheads D in G). The glom117 mutant polypeptide appears to retain this distribution. It is present both in the optic nerve (arrowheads in E and H) and in ectopically localized plexiform patches (arrows in E and H). Western blotting indicates that N-cadherin polypeptide is absent in pactm101b/tm101b mutant embryos (I) but it persists in glom117/m117 animals (J) at 3 dpf. (D, E, G, H) Retinae at 50 hpf. Retina in (F) is at 72 hpf. “le” indicates lens. Arrowheads in (A–C) indicate the midline. In (A–H) dorsal is up.

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Reprinted from Developmental Biology, 259(1), Malicki, J., Jo, H., and Pujic, Z., Zebrafish N-cadherin, encoded by the glass onion locus, plays an essential role in retinal patterning, 95-108, Copyright (2003) with permission from Elsevier. Full text @ Dev. Biol.