Fig. 1

Fig. 1 Injection scheme for the generation of SB transgenic lines. (A, left) Structure of a Sleeping Beauty transposon consisting of flanking IR/DR sequences (gray with orange triangles), a ubiquitous EF1α enhancer/promoter (blue) driving expression of a GFP reporter (green), and terminating with a SV40 poly(A) signal (white). In vitro-transcribed, capped SB10 transposase mRNA. The SB transposon is injected with or without SB10 mRNA into one-cell zebrafish embryos (A, middle), resulting in mosaic GFP expression at 24 hpf (A, far right). (B, left) Each injected transposon vector +/- SB10 mRNA is listed with the corresponding germline transmission and expression frequency, obtained as the percentage of F₀ fish producing GFP-positive offspring. The number of fish screened is shown in parentheses. The F₀ germline mosaicism rate is determined as the percentage of GFP-positive F₁ offspring from a founder outcross. The number of linkage groups harboring a transposon insertion is also listed. For each experiment, embryos with germline integration of ubiquitous GFP expression at 25 hpf in the F₂ (top and bottom) and F₈ (middle) generations are shown under GFP fluorescence detection conditions.