Fig. 1
DAPT Treatment Blocks Notch Signalling Rapidly
DAPT causes loss of her4 expression (A) in the neural tube and (B) in the anterior PSM (where her4 is normally expressed in a faint but detectable stripe). Wild-type embryos were treated with 100 μm DAPT or with DMSO (control) medium for 1 h and stained by ISH for her4. In each panel, the DMSO control is shown on the left, the DAPT-treated embryo on the right. Treatment was begun at the 13-somite stage for (A) and at the 15-somite stage for (B). For each case, at least 14 embryos were examined, and typical specimens were selected for this illustration.
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