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Fig. 5 H-GnRH cells undergo cell division after becoming GnRH-positive. (A–C) Ventral view of 56 h embryo processed for both anti-H3 and anti-GnRH antibodies. (A) H-GnRH cells are dividing (arrow) as are non-H-GnRH cells (asterisk). (B) H-GnRH cells (arrow) are positive for anti-GnRH antibody. TN-GnRH cells are evident out of the plane of focus (arrowhead). (C) Colabeling of the H-GnRH cells for the anti-H3 (arrow) and anti-GnRH antibodies. Scale bar: (A–C) 40 μm.
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Reprinted from Developmental Biology, 257(1), Whitlock, K.E., Wolf, C.D., and Boyce, M.L., Gonadotropin-releasing hormone (gnrh) cells arise from cranial neural crest and adenohypophyseal regions of the neural plate in the zebrafish, Danio rerio, 140-152, Copyright (2003) with permission from Elsevier. Full text @ Dev. Biol.