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Fig. 3 sq181 mutant hepatoblasts are impaired in proliferation and subsequently undergo cell apoptosis that leads to a liverless phenotype. (A,B) Immunostaining using an anti-P-H3 antibody on (A) wild-type (wt) and (B) mutant (mu) zebrafish embryos at 30 hpf. (C,D) TUNEL assay of cell apoptosis in (C) wild-type and (D) mypt1sq181 mutant embryos at 38 hpf. The white dotted line circles the liver primordium and the adjacent endoderm region.
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