Fig. 8

Fig. 8 Localization of EYFP-Il1rapl1b, EYFP-CT swap and EYFP-IL-1RAcP in olfactory sensory neurons and effect of these EYFP-tagged proteins on VAMP2-ECFP punctum formation. (A) Representative VAMP2-ECFP signals (left, green) and EYFP-tagged protein signals (middle, red) in olfactory sensory neurons of Pomp-VC-Y-Il1rapl1b (top)-, Pomp-VC-Y-swap/CT (middle)-, or Pomp-VC-Y-IL1RAcP (bottom)-injected embryos at 60 hpf. These signals are merged on the right. op, olfactory placode; ob, olfactory bulb. Bar = 20 μm. (B) The ratios of the EYFP fluorescent intensity in the olfactory bulb to that in both olfactory placode and olfactory bulb were measured in Pomp-VC-Y-Il1rapl1b (black bar)-, Pomp-VC-Y-swap/CT (crosshatched bar)-or Pomp-VC-Y-IL1RAcP (gray bar)-injected embryos. The ECFP fluorescent intensity ratio in Pomp-VC-injected embryos is also shown (open bar). All values represent mean ± SEM. 8–10 embryos. (C) Representative VAMP2-ECFP signals (left, green) and EYFP-tagged protein signals (middle, red) in the axon terminal of olfactory sensory neuron of Pomp-VC-Y-Il1rapl1b (top)-, Pomp-VC-Y-swap/CT (middle)-or Pomp-VC-Y-IL1RAcP (bottom)-injected embryos at 60 hpf. These signals are merged on the right. Arrowheads point axonal shafts. Bar = 5 μm. (D) The punctate areas of VAMP2-ECFP and EYFP-tagged proteins in axon terminals of olfactory sensory neurons in Pomp-VC-Y-Il1rapl1b (filled circle)-, Pomp-VC-Y-swap/CT (open circle)-and Pomp-VC-Y-IL1RAcP (gray circle)-injected embryos at 60 hpf. n = 35–37, 21 embryos. One-way ANOVA for the punctuate area of VAMP2-ECFP and EYFP-tagged proteins, F = 8.0 and p = 0.001, and F = 2.5 and p = 0.09, respectively. All values represent mean ± SEM. * and **, p < 0.05 and < 0.01, respectively.