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Fig. S2 Induction of gsc expression by cell implantation into the animal pole of embryos. Ectopic expression of gsc is visible around implanted cells despite implantation into the animal pole. (A) Control, non-implanted embryos. (B, C). Implanted embryos. Ectopic gsc was observed in 20 of 23 embryos in ZE6-1 implanted embryos. (C) Whole-mount in situ hybridization for gsc in an implanted embryo. (A–C) Animal pole views.
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Reprinted from Developmental Biology, 321(2), Hashiguchi, M., Shinya, M., Tokumoto, M., and Sakai, N., Nodal/Bozozok-independent induction of the dorsal organizer by zebrafish cell lines, 387-396, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.