|
Fig. 8 Noggin1 and fgf8 induce similar dorsalizations but have different effects on bmp2b expression. (A, D, G) Dorsal view of 5-somite-stage embryos, anterior to the left. (A) Uninjected control embryo. Both nog1 (D) overexpression and fgf8 (G) overexpression induce a dorsalization of the embryo, which displays an ovoid shape and enlarged somites. (B, E, H) Animal pole view of blastula-stage embryos, dorsal to the right. (B) bmp2b expression in a wild-type embryo. (E) bmp2b expression is still present following nog1 overexpression, whereas it is lost following fgf8 overexpression (H). (C, F, I) Lateral view of midgastrula-stage embryos, dorsal to the right. (C) In the wild-type embryo, bmp2b is expressed in the ventral blastoderm (vb), the ventral yolk syncytial layer (arrowhead), and the marginal zone (arrow). (F, I) Following nog1 or fgf8 overexpression, bmp2b expression is lost in the ventral blastoderm and maintained only in the ventral yolk syncytial layer and the marginal zone.
Reprinted from Developmental Biology, 214(1), Fürthauer, M., Thisse, B., and Thisse, C., Three different noggin genes antagonize the activity of bone morphogenetic proteins in the zebrafish embryo, 181-196, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.