|
Fig. 4 Relative expression of U6 maxigene transcripts from plasmids injected into zebrafish embryos. Equal masses of zU4-1maxigene and zU6-1maxigene plasmid DNAs were injected into zebrafish embryos, and total RNA was extracted at the 16-somite stage. Specific transcripts were amplified by RT-PCR, electrophoresed on 18% polyacrylamide gels, stained with ethidium bromide, and relative band intensities were determined. Band intensities from -RT samples (lanes 1, 3, 5, 7) were subtracted from +RT samples (lanes 2, 4, 6, 8), and U6-1maxi signals were normalized to U4-1maxi signals obtained from like RNA samples. Numbers in the row below the lane numbers are the relative percentage amounts of normalized zU6-1maxi RT-PCR product compared to the wt promoter set at 100% (lane 4), and are the average of two independent injection experiments for each plasmid DNA. Numbers in parentheses are the relative expression levels from each experiment.
Reprinted from Gene, 421(1-2), Halbig, K.M., Lekven, A.C., and Kunkel, G.R., Zebrafish U6 small nuclear RNA gene promoters contain a SPH element in an unusual location, 89-94, Copyright (2008) with permission from Elsevier. Full text @ Gene