ZFIN Image: Park et al., 2000, Fig. 3

Image

Figure Caption

Fig. 3 Determination of transcription start site of HuC gene by primer extension. A [γ-³²P]ATP-labeled antisense oligonucleotide primer corresponding to the 24 bp (+169 to +192) of exon-1 of HuC was hybridized with total RNA isolated from 24 hpf zebrafish embryos (Z) or tRNA from yeast (Y) and subjected to primer extension. The extended DNA samples were size-fractionated on a denaturated 6% polyacrylamide gel containing 8 M urea. For the size ladder, a dideoxy DNA sequencing reaction for the zebrafish HuC genomic DNA with the same antisense oligonucleotide primer was performed and then simultaneously electrophoresed with the primer-extended samples on the same gel. An extended cDNA band from zebrafish RNA is indicated by the arrow and the corresponding nucleotide G is marked by an asterisk.

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Reprinted from Developmental Biology, 227(2), Park, H.-C., Kim, C.-H., Bae, Y.-K., Yee, S.-Y., Kim, S.-H., Hong, S.-K., Shin, J., Yoo, K.-W., Hibi, M., Hirano, T., Miki, N., Chitnis, A.B., and Huh, T.-L., Analysis of upstream elements in the HuC promoter leads to the establishment of transgenic zebrafish with fluorescent neurons, 279-293, Copyright (2000) with permission from Elsevier. Full text @ Dev. Biol.