Fig. S29
- ID
- ZDB-IMAGE-080912-16
- Publication
- Kapsimali et al., 2007 - MicroRNAs show a wide diversity of expression profiles in the developing and mature central nervous system
- All Figures
- Figures for Kapsimali et al., 2007
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Fig. S29
Mismatch test for let-7a, miR-92b, miR-153a and miR-181a.
To test the ability of LNA probes to discriminate between different members within a miRNA family, we performed in situ hybridization with single and double mismatch probes for let-7a, miR-92b, miR-153a and miR-181a. In parallel we performed in situ hybridization with the fully matching LNA probes. All probes were labeled and hybridized in parallel.
A,C,E,G: show in situ hybridization signal for full matching LNA probes miR-181a, let-7a, miR-92b and miR-153a, respectively.
B,D,F,H: show in situ hybridization signal for a double mismatch miR-181a probe (MMmiR-181a) and single mismatch probes let-7a, miR-92b and miR-153a (MMlet-7a, MMmiR-92b and MMmiR-153a), respectively.
Although there is a strong reduction in hybridization signal with the single mismatch probes (MMlet-7a, MMmiR-92b and MMmiR-153a), hybridization was only fully eliminated by the double mismatch probe MMmiR-181a. Thus, LNA probes may not be able to fully discriminate between miRNA family members that differ at a single position.