IMAGE

Fig. 3

ID
ZDB-IMAGE-080909-13
Antibodies
Source
Figures for Snow et al., 2008
Image
Figure Caption

Fig. 3 Muscle morphogenesis in fn morphant embryos is more disrupted than in aei/deltaD mutant embryos. A: Summary of experimental design. Muscle development in the posterior of aei/deltaD mutant embryos, where initial somite boundaries did not form, was compared with muscle development in the posterior of 3-ng fn1+3 morphant embryos, where initial somite boundaries did form. Three phenotypes were assessed: uncoupling of fast and slow fiber lengths, absolute fast and slow fiber lengths, and anisotropy (a quantitative measure of organization, see Fig. 4). B-G: Fast-twitch and slow-twitch fiber lengths are more frequently uncoupled in fn morphant embryos than in aei/deltaD mutant embryos. Side views, anterior left, F59 (green) denotes slow-twitch muscle and phalloidin (red) denotes fast-twitch muscle. B,C: Fast-twitch and slow-twitch fibers are approximately the same length at 69% of boundaries in fn1+3 morphant embryos, and at 92% of boundaries in aei/deltaD morphant embryos. D,E: When slow-twitch fibers exhibit disruptions such as gaps, fast-twitch fibers are longer than slow-twitch fibers. F,G: Fast-twitch fibers are sometimes longer than slow-twitch fibers when slow-twitch fibers either appear normal or show only minor disruptions. H: Fast-twitch fibers are longer in fn1+3 morphant embryos than in aei/deltaD mutant embryos. H1-H2: Fibers were interactively traced in Axiovision and the major axes calculated. Note that some fibers are unusually long (white arrowhead). H3,H4: Average fast-twitch and slow-twitch fiber lengths are significantly longer in fn morphant embryos than in either control or aei/deltaD mutant embryos (P < 0.01). H5: The ratio of fast-twitch to slow-twitch fiber lengths is higher in fn1+3 morphant embryos. This increase is indicative of the more frequent uncoupling of fast-twitch and slow-twitch fiber lengths in fn morphant embryos.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.