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Fig. S2 Evidence of the specificity of Cre activity in Tg(zlmo2:Cre) transgenic line. beta-actin-loxP-DsRed-loxP-EGFP reporter plasmid was injected into the Tg(zlmo2:Cre) embryos. A,B: Relatively ubiquitous expression of DsRed at 18 hpf and 22 hpf. C,D: Expression of EGFP in the head vasculature and ICM at 18 hpf and 22 hpf (arrows). All embryos are shown with heads to the left.
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