Fig. 1

Fig. 1 Expression of lpp during zebrafish development. (A) Expression of lpp and β-actin mRNA as determined by RT-PCR at different stages of zebrafish development and in non-fertilized eggs (NF). nc, negative control. (B–N) Whole-mount in situ hybridization analyses for zebrafish lpp expression at different stages of embryonic development using an RNA probe against the 3′UTR of lpp. (B) 16-cell stage, animal pole view. (C) dome stage, animal pole view. (D) shield stage, animal pole view, dorsal to the right. (E) tailbud stage, lateral view, dorsal to the right. (F) tailbud stage, dorsal view, anterior on top. (G) tailbud stage, vegetal pole view. (H) 14-somite stage, lateral view, dorsal to the right. (I) 14-somite stage, dorsal view. (J) Prim-5 stage, head region, dorsal view. (K) Prim-5 stage, lateral view. (L) Prim-5 stage, dorsal view. (M) Long-pec stage, lateral view. (N) Long-pec stage, frontal view. Abbreviations: nc, notochord; tb, tailbud; isv, intersomitic vessels; h, heart.