Fig. S1

Fig. S1 Testing the LexPR transcription control using RT-PCR. RT-PCR was performed on RNA extracted from transgenic F₂ embryos carrying the driver-reporter construct using two pairs of primers to detect EGFP (top panel) and LexPR transactivator (bottom panel) RNA products. Embryos (36 hpf) were treated with 1μM mifepristone for 12 hours (48hpf). After that, the embryos were rinsed and transferred into larger tanks without mifepristone. Six mRNA samples were prepared and tested: 1) before induction (36 hpf); 2) after 1-hour induction; 3) after 12-hours induction; 4) 1 day after mifepristone withdrawal; 5) 5 days after mifepristone withdrawal; 6) wild type - negative control. The PCR-conditions (number of cycles) were optimized for detection of low quantities of RNA target, not for quantitative comparison between samples. Thus, there is in no visible product downregulation at the lane 5 (5 days post withdrawal).