Fig. 8 Immunostaining of 48 hour post-fertilization zebrafish embryos with anti-VDR antibody or pre- immune serum. 100 X original magnification. Panel A. Coronal section. 48 hour post-fertilization embryo immunostained with anti-VDR antibody. Note staining (brown color) of cells within the eye (neural retina), the brain (diencephalon) and the developing mandible. 100 X original magnification. Panel B. Sagittal section. 48 hour post-fertilization embryo immunostained with immune serum. Note staining of cells of the neural retina, brain and developing mandible. Panel C. Coronal section. 48 hour post-fertilization embryo immunostained with pre-immune serum. Note absence of staining. 100 X original magnification. Panel D. Sagittal section. 48 hour post-fertilization embryo immunostained with pre-immune serum. Note absence of staining in cells of the neural retina, brain and developing mandible.
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Reproduced from J Bone Miner Res 2008;23:1486-1496 with permission of the American Society for Bone and Mineral Research
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