Fig. 4 DNA polymorphisms generated by DANA-specific primers in PCR. 32P-labeled PCR amplification products were separated in polyacrylamide gels. (A) Effect of the specificity and combination of PCR primers on the number of DNA fragments amplified from Singapore zebrafish DNA. Lanes: 1, Dana-1 primer only; 2, Dana-1 supplemented with two TdrI-specific primers, TdrA and FTC-12. (B) Polymorphic patterns generated by DANA-PCR using the same primer combination as in lane 2 of A, on DNA samples from different zebrafish populations. Lanes: 1, Hong Kong; 2, Indonesia; 3, Singapore; 4, wild-type 1 (United States); 5, wild-type 2 (United States); 6, “gold” zebrafish; 7, clonal line C-32; 8, “leopard”/C-32.
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