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Fig. 2

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ZDB-IMAGE-080701-36
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Figures for McFarland et al., 2008
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Fig. 2 Identification of cerebellar cell types reveals distinct populations of olig2+ neurons organized along the DV axis. (A–C) Sagittal section through cerebellum of 48 hpf Tg(olig2:egfp) transgenic embryo showing EGFP+ cells expressed Hu (red). Arrowheads mark long axonal projections. (D) Dorsal view with anterior to left of one-half of the cerebellum in living Tg(olig2:egfp) embryo at 56 hpf. Bracket and arrowheads mark EGFP+ axons extending from EGFP+ cells ventrally along midbrain–hindbrain boundary. (E) Dorsal view of cerebellum in living Tg(olig2:egfp) larva at 7 dpf, anterior is along the bottom. Bracket indicates extensive dendritic branches originating from EGFP+ cells. (F) 3-D reconstruction of 5 dpf sagittal section of a Tg(olig2:egfp) larva illustrating DV organization of Zebrin II+ (green), EGFP+ (blue) and Calretinin+ (red) cells. Arrowhead marks ventrally extending EGFP+ axons. (G) Transverse section a 5 dpf Tg(olig2:egfp) larva showing Zebrin II, EGFP and Calretinin labeling of distinct populations and DV arrangement. (H–J) Sections of 14 dpf Tg(olig2:egfp) transgenic larvae labeled with Calretinin, Parvalbumin and Zebrin II antibodies. (H) Transverse hemisection shows EGFP+ cells dorsal to Calretinin+ eurydendroid cells. (I) Transverse hemisection showing EGFP+ cells intermixed with Zebrin II+ Purkinje neurons. Inset shows enlargement of area framed by dashed lines. (J) Sagittal section showing distinct populations of Parvalbumin+ Purkinje neurons and EGFP+ neurons. Arrowheads mark EGFP+ axons extending toward deep brainstem. Inset shows enlargement of area framed by dashed lines. Scale bar = 20 μm for all panels except panel G, for which it represents 40 μm.

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Reprinted from Developmental Biology, 318(1), McFarland, K.A., Topczewska, J.M., Weidinger, G., Dorsky, R.I., and Appel, B., Hh and Wnt signaling regulate formation of olig2(+) neurons in the zebrafish cerebellum, 162-171, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.