Fig. S5 Inhibition of cytokinesis does not interfere with perinuclear localization of granules following nuclear division. Epifluorescence pictures of germ cells labeled with Farnesylated-GFP, H1M-GFP for visualizing plasma membrane, and Vasa-dsRed for germ cell granules. A) Cells expressing RhoAN19 do not undergo cytokinesis and polynucleated cells are observed. Although no cytokinetic ring is formed, granules reach the nuclear envelope (n) and readopt perinuclear localization (arrowhead) after division of the nucleaus. B) Interphase cells show normal perinuclear distribution of germ cell granules in both nuclei.
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