ZFIN Image: Fadool et al., 1998, Fig. 2

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Figure Caption

Fig. 2 PCR amplification of the mariner element in F₁ progeny. (a) Genomic DNA was isolated from pools of 20-30 F₁ generation larvae from six pairwise matings of founder fish (numbered 1-6) and used as template for amplification of mariner sequences. Nine of the pools of larvae from four different crosses tested positive for the transgene as indicated by a product at 450 bp (arrow). (b) Analysis of DNA samples isolated from fin clippings of individual F₁ offspring from mating of founder pair number 5. Genomic DNA from 2 of the 15 individuals demonstrated the presence of mariner sequences (arrow) (lane M, 100-bp marker; Promega; lane B, no DNA control; lane C, positive control containing 1 ng pWE1 plasmid).

Acknowledgments

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