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Fig. 2 tiy121 is an emi1 mutant. (A) The chromosomal location of tiy121/emi1. (B) tiy121 is a premature stop codon in emi1. NLS, nuclear localization signal. (C) Graph of the levels of mitosis in tiy121, emi1 morphants, and tiy121 embryos rescued by injection of emi1 mRNA. The level of mitosis, graphed along an arbitrary scale, was wild-type (wt) or reduced to some degree, or mitosis was absent. Morphants and mRNA-injected embryos develop normally prior to the shield stage, thus we infer that the initial level of mitosis is normal without examining PHH3 (dashed lines). hpf, hours post fertilization. (D) tiy121 and hi2648, an insertional allele of emi1, do not complement. (E-G) Expression of emi1 mRNA (E) at the one-cell stage, (F) at the sphere stage, and (G) in the most recently formed somites (arrowheads) and anterior PSM of a 12-somite stage embryo. Anterior is left in G. (H) YFP-Emi1 immunofluorescence. (I) DAPI stained nuclei. (J) Overlay of H and I. Arrows indicate a cell completing mitosis. Scale bar in H: 20 μm for H-J.