Fig. 10

Fig. 10 Expression of gdnf in the intestinal mesenchyme requires endoderm and HH signaling but not hand2 function. (A, F, K) wildtype control embryos, (B, G, L) hand2 morphants (C, H, M) cas/sox32 morphant embryos, (D, I, N) 24–26 hpf cyclopamine treated embryos and (E, J, O) 36–60 hpf cyclopamine treated embryos. (A–E) Ventral views of 36 hpf wholemount embryos that have been hybridized with a gdnf antisense probe and the yolk removed. In sox32 morphant (C) and 24–36 hpf cyclopamine treated embryos (D) there is a loss of gdnf expression at the anterior end of the intestine. (F–J) Lateral view of 60 hpf wholemount embryos, that have been hybridized with a gdnf antisense probe, showing the loss of intestinal gdnf expression in sox32 morphants and cyclopamine treated embryos. (K–O) Lateral view of the intestine of 60 hpf wholemount embryos that have been hybridized with a gdnf antisense probe. Arrows (A, B, E) indicate gdnf expression at the anterior end of the intestine. Arrow heads (K, L) indicate the gdnf expression along the length of the intestine. Anterior is to the left.