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Fig. 2 Germ-line replacement strategy using the rhodamine-dextran labeling technique. (A) Overview of transplantation strategy showing the transfer of cells from the margin of rhodamine-dextran-labeled mutant donor embryos into the animal pole of αPGC-MO-injected WT hosts. (B) At 30 hpf, all cells within control donor embryos are efficiently labeled with rhodamine-dextran. (C) Chimeric host embryo at 30 hpf showing somatic contribution of rhodamine-dextran-labeled donor cells to anterior neuroectoderm lineages (*). Host embryos were screened for the presence of labeled donor PGCs that had migrated successfully into the gonadal mesoderm (arrow).