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Fig. 1 Developmental expression of gelsolin mRNA. Gelsolin mRNA expression is shown in four stages of embryo by using an antisense probe: two-cell (a), four-cell (c), blastula (e), and late-gastrula (f). A sense probe was used to hybridize in the two-cell (b) and four-cell (d) embryos. The gastrula (e) is photographed, magnified x2 to show the anterior part of the embryo. Gelsolin expression in the forebrain and hindbrain is shown in a 22-h-old embryo (g), in the snout and cornea of a hatching-stage embryo (h), and in the sections of eyes from a hatching-stage embryo probed with the sense (i) and antisense (j) riboprobes. Arrow indicates the location of the eye. Locations of the lens (L) and cornea (C) are indicated. (k) RT-PCR for gelsolin by using cDNA synthesized from embryonic RNA obtained from the indicated developmental stages. (Upper) Reaction products with reverse transcriptase (+RT). (Lower) Products without the enzyme (–RT).