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Fig. 3 Cre-mediated recombination in transiently injected embryos. (A) Diagram of the CMV-loxP-dsRED2-loxP-EGFP construct. (B-G) One-cell-stage embryos were injected with the CMV-loxP-dsRED2-loxP-EGFP vector in the absence of Cre RNA (- Cre RNA) (B-D) or with Cre RNA (+ Cre RNA, 25 ng/μl) (E-G). Shown are bright-field (B and E), red fluorescence (dsRED2) (C and F), and green fluorescence (EGFP) images (D and G) of embryos at 26 h postfertilization. Anterior is to the left, and dorsal is toward the top.
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