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Figure Caption
Fig. 6 Knockdown of functional insulin-like growth factor-binding protein (IGFBP)-1 does not change the expression levels or splicing of other IGFBPs. RT-PCR was performed with specific primer sets for zebrafish IGFBP-2, IGFBP-3, and IGFBP-5 by using RNA isolated from WT, control MO (Cont), and various doses of IGFBP-1 targeting MO1-injected (0.5, 1, 2, 4, 8, and 16 ng) embryos as described in Materials and Methods.
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