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Fig. 5 Rescue of zebrafish cranial motor neuron migration defects with human TBX20 mRNA. (A, B) 48 hpf tg(isl1:GFP) zebrafish embryos. (A) Uninjected control. (B) Embryo treated with 2 ng of tbx20 morpholino. (C–G) Embryos injected with 2 ng of tbx20 morpholino show rescue of the neuronal migration phenotype following injections of mRNA (from 50 to 250 pg/embryo increasing from panels C to G in 50 pg increments) encoding HsTBX20. Abnormal migration of nV and nVII motor neuron soma visualized in tg(islet1::GFP) embryos (B) is reverted by co-injection of mRNA for HsTBX20. (H) Percentage of tg(isl1:GFP) embryos (Y axis) showing normal migration of nVII motor neurons following co-injection of increasing (1, 2, 4, 6 ng) doses of tbx20 Mo (X axis) with 100 pg mRNA encoding HsTBX20.
Reprinted from Developmental Biology, 317(2), Pocock, R., Mione, M., Hussain, S., Maxwell, S., Pontecorvi, M., Aslam, S., Gerrelli, D., Sowden, J.C., and Woollard, A., Neuronal function of Tbx20 conserved from nematodes to vertebrates, 671-685, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.