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Fig. 1 Whole mount zebrafish embryos processed for in situ hybridization using cmlc2 (A, C, E) or ndrg4 (B, D, F–M) specific anti-sense RNA probes. cmlc2 is expressed in the bilateral cardiac primordia at the 14-somite stage (A), in the cardiac cone at the 21-somite stage (C) and in the heart tube at the 24 hpf (E). The first significant indication of ndrg4 cardiac expression occurs in the heart tube (arrow) at 24 hpf (F) and at 33 hpf (G). ndrg4 is robustly expressed in both atrium and ventricle at 36 hpf (H), 48 hpf (I–L) and 72 hpf (M) with stronger signal in the ventricle. (J) Transverse section showing strong expression of ndrg4 in the ventricle at 48 hpf. (L) Higher magnification of the indicated heart region in panel K. (A–D) Dorsal views with anterior towards the top; (E–G, K, L) lateral views; (H, I, M) frontal views with anterior towards the top. a, atrium; v, ventricle. Arrow indicates heart or heart region.
Reprinted from Developmental Biology, 317(2), Qu, X., Jia, H., Garrity, D.M., Tompkins, K., Batts, L., Appel, B., Zhong, T.P., and Baldwin, H.S., ndrg4 is required for normal myocyte proliferation during early cardiac development in zebrafish, 486-496, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.