Detection of the MYC-ADMP protein. (Top panel) myc-admp RNA and nls-gfp RNA (50 pg) were injected at the 16-cell stage into 1 marginal blatomere, and MYC-ADMP expression was revealed by immunocytochemistry at the sphere stage. NLS-GFP was used as a lineage tracer to identify the progeny of the injected cell. Embryos where observed with a confocal microscope. (A–C) myc-admp RNA (200 pg) (B)-injected embryo (10x objective); white boxes indicate a GFP-positive region and a GFP-negative region showed at a higher magnification in (A) and (C), respectively. (A, C, G) 63x objective. (A) Injected embryo, GFP-positive domain. (C) Injected embryo, GFP-negative domain. (G) Uninjected embryo. (D–F) 40x objective. (D–E) myc-admp (50 pg) and nls-gfp RNA (50 pg) (E) admp-morpholino (500μM) was injected at the one-cell stage prior to myc-admp and nls-gfp RNA injection at the 16-cell stage. (F) Uninjected embryo. (Bottom panel) myc-admp RNA (1μg) or no RNA (C) was translated in vitro in the absence (0) or in the presence of admp-morpholino (10, 2, or 0.4 μg in a reaction volume of 25 μl). The synthesis of MYC-ADMP protein (45 kDa, arrowhead) was inhibited by the admp-morpholino (admp-Mo) in a dose-dependent manner, and was not affected by the presence of a control morpholino (Co-Mo).
ZFIN wishes to thank the journal for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Your Input Welcome
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if
additional information is required.
Oops. Something went wrong. Please try again later.