ZFIN Image: Shepherd et al., 2001, Fig. 5

Image

Figure Caption

Fig. 5 Focal overexpression of GDNF in zebrafish somitic muscle cells causes PMN axon pathfinding errors. (A–C) Lateral view of the trunks of 24 hpf antiacetlyated tubulin antibody stained control (A), focal GDNF overexpressing embryos (B), and uniform GDNF overexpressing embryos (C). Red cells in (B) and (C) express the muscle actin promoter: GDNF DNA construct as revealed by whole-mount in situ hybridization with a riboprobe against a 3′ UTR tag contained in the injected DNA construct. Arrow in (B) indicates a stalled PMN axon projection associated with a GDNF overexpressing cell. Scale bar: 50 μm.

Acknowledgments

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Reprinted from Developmental Biology, 231(2), Shepherd, I.T., Beattie, C.E., and Raible, D.W., Functional analysis of zebrafish GDNF, 420-435, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.