Fig. 5 Cyclin B mRNA in full-grown oocytes treated with 1 (A, B) or 10 μg/ml (C) cytochalasin B. In the oocytes treated with 1 μg/ml cytochalasin B, the aggregation of cyclin B mRNA is seen as various forms depending on batches of oocytes (the aggregation was segregated into pieces scattered in the cytoplasm as in A or only deformed without apparent segregation as in B), whereas it was completely dispersed in the oocytes treated with 10 μg/ml cytochalasin B (C). Note that 10 μg/ml cytochalasin B induced GVBD in situ without migration of the germinal vesicle (indicated by arrow). GV, germinal vesicle (marked by broken lines). Scale, 100 μm.
Reprinted from Developmental Biology, 229(2), Kondo, T., Kotani, T., and Yamashita, M., Dispersion of cyclin B mRNA aggregation is coupled with translational activation of the mRNA during zebrafish oocyte maturation, 421-431, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.