ZFIN Image: Ryu et al., 2001, Fig. 4

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Figure Caption

Fig. 4 The dharma promoter can be regulated by Wnt but not Nodal signal. (A) pDha-1420 GFP plasmid DNA was coinjected with synthetic RNAs for wnt8b (200 pg), dvl3 (520 pg), DN gsk3β (480 pg), GBP (480 pg), ΔNβ-catenin (100 pg), ΔNβ-catenin (100 pg), and DN Tcf3 (100 pg) as indicated. Expression of GFP was detected at the 30% epiboly stage except for DN GSK3β RNA-injected embryos (sphere stage). (B) pDha-1420 GFP plasmid DNA was coinjected with synthetic RNAs for squint (1 pg) and antivin (5 pg) as indicated. Expression of GFP was detected at the shield stage. The squint RNA- and antivin RNA-injected embryos displayed a severely dorsalized phenotype and a cyclops;squint-like phenotype at pharyngula period, respectively (data not shown). Lateral view and dorsal is to the right.

Acknowledgments

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Reprinted from Developmental Biology, 231(2), Ryu, S.-L., Fujii, R., Yamanaka, Y., Shimizu, T., Yabe, T., Hirata, T., Hibi, M., and Hirano, T., Regulation of dharma/bozozok by the Wnt Pathway, 397-409, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.