Fig. 1 Regulation of dharma by Wnt signals. Synthetic RNAs for Wnt8b (200 pg), Dvl3 (520 pg), DN GSK3β (480 pg), an activated form of β-catenin (ΔNβ-catenin, 100 pg), GBP (480 pg), mNLK (100 pg), or DN Tcf3 (100 pg) were injected into one-cell-stage embryos. For yolk depletion, the vegetal yolk mass was removed at the one-cell stage. The embryos were fixed at the sphere stage (4 hpf) and the dharma transcripts were detected by whole-mount in situ hybridization. (A) In situ data representing each condition. (B) The proportions of embryos displaying reduced, normal, and expanded expression of dharma are shown as percentages.
Reprinted from Developmental Biology, 231(2), Ryu, S.-L., Fujii, R., Yamanaka, Y., Shimizu, T., Yabe, T., Hirata, T., Hibi, M., and Hirano, T., Regulation of dharma/bozozok by the Wnt Pathway, 397-409, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.