ZFIN Image: Gray et al., 2001, Fig. 11

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Figure Caption

Fig. 11 Notch rescues the des^b420 Mauthner cell defect. Embryos from wild-type or heterozygous desb420 matings were injected at the 1- to 2-cell stage with RNA encoding Notch-ICD-myc (Coffman et al., 1993; Chitnis et al., 1995). Embryos were fixed at 28 h and processed for 3A10 antibody labeling to visualize Mauthner cells. (A) Mock-injected wild-type embryos have one Mauthner cell on either side of hindbrain rhombomere 4. (B) Approximately 25% of mock-injected embryos from a heterozygous des^b420 mating have supernumerary Mauthner cells. Injecting RNA encoding Notch-ICD decreases the number of Mauthner cells in wild-type (C) and des^b420 mutant (D) embryos.

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Reprinted from Developmental Biology, 237(2), Gray, M., Moens, C.B., Amacher, S.L., Eisen, J.S., and Beattie, C.E., Zebrafish deadly seven functions in neurogenesis, 306-323, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.