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Fig. 8 EnR-pitx2 interferes with specification of the mesendoderm. Embryos were injected vegetally into each blastomere at the four-cell stage with 50, 100, or 200 pg of EnR-pitx2 mRNA, collected at stage 11, and analyzed by RT-PCR for the expression of sox-17β, mixer, GATA-4, and edd (panendodermal markers); dkk-1 and cerberus (anterior endomesoderm markers); Xbra (general mesoderm); chordin (dorsal mesoderm); Xanf-1 and Xanf-2 (ectodermal markers at this stage); siamois and Xnr-1 (dorsal endoderm); and histone H4 (loading control).
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Reprinted from Developmental Biology, 229(2), Faucourt, M., Houliston, E., Besnardeau, L., Kimelman, D., and Lepage, T., The pitx2 homeobox protein is required early for endoderm formation and nodal signaling, 287-306, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.